USP HPLC Packings – Develosil Columns

USP listing table

Tip: Use this table for regulatory documentation, method transfer, and selecting an equivalent chemistry when a method specifies only a USP L packing.

What the USP packings mean for Develosil selection

USP L1 – Develosil ODS

USP definition: Octadecyl silane chemically bonded to porous or non-porous silica or ceramic micro-particles, 1.5 to 10 µm in diameter, or a monolithic rod.

Develosil ODS (C18) is the most common reversed‑phase starting point for routine assay and impurity profiling. Choose this when you want broad applicability, strong hydrophobic retention, and robust method performance across many compound classes.

• Best for: general RP separations, pharma QC, stability studies
• Method transfer: match L1 chemistry first, then match particle size and dimensions
• When to switch: if you need more polar retention (try aqueous‑compatible RP) or different selectivity (CN, phenyl, etc.)

USP L10 – Develosil CN‑UG

USP definition: Nitrile groups chemically bonded to porous silica particles, 1.5 to 10 µm in diameter, or a monolithic silica rod.

Develosil CN‑UG (cyano) provides a useful selectivity change versus C18. It can be operated in reversed‑phase and, in some workflows, normal‑phase mode depending on mobile phase selection. Choose CN when you need a mid‑polarity surface and orthogonal selectivity for method development.

• Best for: isomer separations, moderately polar analytes, orthogonal RP screening
• Typical benefit: different elution order compared to ODS, improved separation for certain aromatic/polar compounds

USP L13 – Develosil TMS‑UG C1

USP definition: Trimethylsilane chemically bonded to porous silica particles, 3 to 10 µm in diameter.

Develosil TMS‑UG C1 is a low‑hydrophobicity (short‑chain) reversed‑phase. It reduces retention for strongly hydrophobic compounds and supports faster run times when C18 over‑retains.

• Best for: fast analysis of non‑polar compounds, reducing excessive retention
• Method transfer: useful when a method needs a “weaker” RP phase than C18

USP L62 – Develosil C30 family and related phases

USP definition: C30 silane bonded phase on a fully porous spherical silica, 3 to 15 µm in diameter.

USP L62 corresponds to C30 chemistry used for enhanced shape selectivity. Develosil C30 phases are widely used for carotenoids, lipid isomers, and other geometrical or positional isomers where C18 may not provide enough selectivity.

• Develosil C30: primary C30 selection for isomeric separations
• Develosil C30‑UG: UG series variant for consistent performance and stability
• Develosil RP‑Aqueous / RP‑Aqueous‑AR: aqueous‑compatible reversed‑phase options when methods require high water content and stable retention for polar analytes
• Develosil Combi‑RP: hybrid selectivity RP option for method development and peak‑shape improvements in challenging matrices